Journal: Cell reports

Article Title: History-dependent dopamine release increases cAMP levels in most basal amygdala glutamatergic neurons to control learning

doi: 10.1016/j.celrep.2022.110297

Figure Lengend Snippet: Key resources table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Virus strains AAV2/1.hSyn.FLEX.GCaMP6s.WPRE Addgene CAT# 100845 AAV2/1.hSyn.DIO.GreenDownward-cADDis.WPRE Boston Children’s Hospital Viral Core Zhang et al., 2021b AAV2/1.hSyn-dLight1.1.WPRE Boston Children’s Hospital Viral Core Lutas et al., 2019 AAV2/1.EF1a.DIO.mKate2-biPAC.WPRE Boston Children’s Hospital Viral Core Zhang et al., 2021b AAV2/1.EF1a.DIO.mKate2-PDE4D3-cat.WPRE Boston Children’s Hospital Viral Core Zhang et al., 2021b AAV2/9.hSyn.FLEX.ExRai-AKAR2-CW3SL Richard Huganir Zhang et al., 2021a AAV2/1.hSyn.FLEX.Chrimson-tdTomato UNC Vector Core Chemicals, peptides, and recombinant proteins SCH23390 hydrochloride Tocris Cat# 0925 {"type":"entrez-protein","attrs":{"text":"SKF81297","term_id":"1156277425","term_text":"SKF81297"}} SKF81297 hydrobromide Tocris Cat# 1447 Dopamine hydrochloride Tocris Cat# 3548 Forskolin Tocris Cat# 1099 IBMX Tocris Cat# 2845 Experimental models: Organisms/strains Mouse: EMX1-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:005628 Mouse: DAT-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:020080 Software and algorithms MATLAB R2019 Mathworks https://www.mathworks.com/products/matlab.html; RRID: SCR_001622 ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Scanbox Neurolabware https://scanbox.org/ Other 10x, 0.5 NA objective ThorLabs CAT# TL10X-2P 4x, 0.28 NA objective Olympus XLFUOR 16x, 0.8 NA objective Nikon CFI75 Two-photon microscope Neurolabware http://neurolabware.com/ InSight X3 laser Spectra-Physics InSight X3 Open in a separate window Key resources table Basal amygdala cAMP signaling facilitates learning of motivational salience of cues Axonal dopamine release increases cAMP in most basal amygdala glutamatergic neurons Presynaptic depression of dopamine release limits postsynaptic cAMP accumulation cAMP responses depress following clustered appetitive or aversive stimuli

Techniques: Plasmid Preparation, Recombinant, Software, Microscopy